کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1903091 | 1534438 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mussel oligopeptides protect human fibroblasts from hydrogen peroxide (H2O2)-induced premature senescence
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کلمات کلیدی
Sirt13-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromidePRX1SAHFGSHPSCΔΨmNAMPTsirtuin 1SA-β-galDMSO - DMSOMTT - MTTsenescence-associated beta-galactosidase - بتا گالاکتوزیداز مرتبط با پیریDimethylsulfoxide - دیمتیل سولفواکسیدnicotinamide phosphoribosyltransferase - نیکوتین آمید فسفریبوسیل ترانسفرازMolecular weight - وزن مولکولیMitochondrial transmembrane potential - پتانسیل ترانزیتی میتوکندریPeroxiredoxin 1 - پراکسیردوکسین 1Premature senescence - پیری زودرسsenescence-associated heterochromatin foci - کانون های هتروکروماتین مرتبط با پیریGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mussel bioactive peptides have been viewed as mediators to maximize the high quality of life. In this study, the anti-aging activities of mussel oligopeptides were evaluated using H2O2-induced prematurely senescent MRC-5 fibroblasts. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry displayed that exposure to H2O2 led to the loss of cell viability and cell cycle arrest. In addition, H2O2 caused the elevation of senescence-associated-β-galactosidase (SA-β-gal) activity and formation of senescence-associated heterochromatin foci (SAHF). It was found that pretreatment with mussel oligopeptides could significantly attenuate these properties associated with cellular senescence. Mussel oligopeptides also led to the increase of glutathione (GSH) level and mitochondrial transmembrane potential (ÎÏm) recovery. In addition, mussel oligopeptides resulted in an improvement in transcriptional activity of peroxiredoxin 1 (Prx1), nicotinamide phosphoribosyltransferase (NAMPT) and sirtuin 1 (SIRT1). This study revealed that mussel oligopeptides could protect against cellular senescence induced by H2O2, and the effects were closely associated with redox cycle modulating and potentiating the SIRT1 pathway. These findings provide new insights into the beneficial role of mussel bioactive peptides on retarding senescence process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Gerontology and Geriatrics - Volume 58, Issue 2, MarchâApril 2014, Pages 293-299
Journal: Archives of Gerontology and Geriatrics - Volume 58, Issue 2, MarchâApril 2014, Pages 293-299
نویسندگان
Yue Zhou, Ying Dong, Qing-Gang Xu, Shu-Yun Zhu, Shi-Lei Tian, Jing-jing Huo, Ting-Ting Hao, Bei-Wei Zhu,