کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1908559 1534986 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deletion of the mitochondrial Pim1/Lon protease in yeast results in accelerated aging and impairment of the proteasome
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Deletion of the mitochondrial Pim1/Lon protease in yeast results in accelerated aging and impairment of the proteasome
چکیده انگلیسی

The Saccharomyces cerevisiae homolog of the ATP-dependent Lon protease, Pim1p, is essential for mitochondrial protein quality control, DNA maintenance, and respiration. Here, we demonstrate that Pim1p activity declines in aging cells and that Pim1p deficiency shortens the replicative life span of yeast mother cells. This accelerated aging of pim1Δ cells is accompanied by elevated cytosolic levels of oxidized and aggregated proteins, as well as reduced proteasome activity. Overproduction of Hsp104p greatly diminishes aggregation of oxidized cytosolic proteins, rescues proteasome activity, and restores life span of pim1Δ cells to near wild-type levels. Our results show that defects in mitochondrial protein quality control have global intracellular effects leading to the increased generation of misfolded proteins and cytosolic protein aggregates, which are linked to a decline in replicative potential.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 56, March 2013, Pages 9–16
نویسندگان
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