کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1908940 1046694 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
8-Oxoguanine-DNA glycosylase 1 deficiency modifies allergic airway inflammation by regulating STAT6 and IL-4 in cells and in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
8-Oxoguanine-DNA glycosylase 1 deficiency modifies allergic airway inflammation by regulating STAT6 and IL-4 in cells and in mice
چکیده انگلیسی

8-Oxoguanine-DNA glycosylase (OGG-1) is a base excision DNA repair enzyme; however, its function in modulating allergic diseases remains undefined. Using OGG-1 knockout (KO) mice, we show that this protein affects allergic airway inflammation after sensitization and challenge by ovalbumin(OVA). OGG-1 KO mice exhibited less inflammatory cell infiltration and reduced oxidative stress in the lungs after OVA challenge compared to WT mice. The KO phenotype included decreased IL-4, IL-6, IL-10, and IL-17 in lung tissues. In addition, OGG-1 KO mice showed decreased expression and phosphorylation of STAT6 as well as NF-κB. Down-regulation of OGG-1 by siRNA lowered ROS and IL-4 levels but increased IFN-γ production in cultured epithelial cells after exposure to house dust mite extracts. OGG-1 may affect the levels of oxidative stress and proinflammatory cytokines during asthmatic conditions. OGG-1 deficiency negatively regulates allergen-induced airway inflammatory response.

Figure optionsDownload high-quality image (56 K)Download as PowerPoint slideHighlight
► OGG1-/- mice exhibit decreased airway inflammatory cells;
► OGG1-/- mice show decrease in IL-4 but increase in INF-γ;
► OGG1-/- mice have lowered oxidative stress;
► OGG1 plays regulatory roles in ovalbumin-induced asthma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 52, Issue 2, 15 January 2012, Pages 392–401
نویسندگان
, , , , , , , , , ,