کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1909810 1046741 2009 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Treatment of human cancer cells with selenite or tellurite in combination with auranofin enhances cell death due to redox shift
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Treatment of human cancer cells with selenite or tellurite in combination with auranofin enhances cell death due to redox shift
چکیده انگلیسی

Selenium is an essential trace element incorporated as selenocysteine in 25 human selenoproteins. Among them are thioredoxin reductases (TrxR) and glutathione peroxidases, all central proteins in the regulation of the cellular thiol redox state. In this paper the effects of selenite and tellurite treatment in human cancer cells are reported and compared. Our results show that both selenite and tellurite, at relatively low concentrations, are able to increase the expression of mitochondrial and cytosolic TrxR in cisplatin-sensitive (2008) and -resistant (C13⁎) phenotypes. We further investigated the cellular effects induced by selenite or tellurite in combination with the specific TrxR inhibitor auranofin. Selenite pretreatment induced a dramatic increase in auranofin cytotoxicity in both resistant and sensitive cells. Investigation of TrxR activity and expression levels as well as the cellular redox state demonstrated the involvement of TrxR inhibition and redox changes in selenite and auranofin combined action.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 47, Issue 6, 15 September 2009, Pages 710–721
نویسندگان
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