کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910455 | 1046772 | 2009 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Detection of protein S-nitrosylation with the biotin-switch technique Detection of protein S-nitrosylation with the biotin-switch technique](/preview/png/1910455.png)
Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in organisms from bacteria to humans, and recent efforts have greatly advanced our scientific understanding of how this redox-based modification is dynamically regulated during physiological and pathophysiological conditions. The focus of this review is the biotin-switch technique (BST), which has become a mainstay assay for detecting S-nitrosylated proteins in complex biological systems. Potential pitfalls and modern adaptations of the BST are discussed, as are future directions for this assay in the burgeoning field of protein S-nitrosylation.
Journal: Free Radical Biology and Medicine - Volume 46, Issue 2, 15 January 2009, Pages 119–126