کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910488 | 1046773 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mutated glutathione-S-transferase reduced airway inflammation by limiting oxidative stress and Th2 response
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کلمات کلیدی
TBARSMGSTCDNBOVATBAPBS1-chloro-2,4-dinitrobenzene - 1-کلرو-2،4-دینیتروبنزنROS - ROSAsthma - آسمThiobarbituric acid - اسید تیوباربیتوریکPeriodic Acid Schiff - اسید فسفریکAirway inflammation - التهاب راه های هواییsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدOvalbumin - اوبلبومینBALF - بافتOxidative stress - تنش اکسیداتیوBronchoalveolar lavage fluid - مایع لارو برونکلوفلورPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMice model - مدل موشPAS - نهglutathione S-transferase - گلوتاتیون S-ترانسفرازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Oxidative stress is an important factor in the pathogenesis of asthma. Furthermore, antioxidants like GST are reduced in asthma patients. In the present study, the therapeutic effects of exogenous GST and mGST were evaluated in a mice model. GST mutated at residues 21/27 has reduced IgE binding with similar enzyme activity as that of GST. To evaluate the therapeutic effects of GST, BALB/c mice were immunized and challenged with ovalbumin. Mice were given GST, mGST, and α-lipoic acid by inhalation and sacrificed on Day 31 to evaluate inflammation and oxidative stress. Mice treated with mGST showed significantly reduced total cell count (P < 0.01) and eosinophils (P < 0.01) in BALF compared to GST- or PBS-treated groups. The lung inflammation score was lowest for the mGST-treated group along with reduced IL-4 (P < 0.01) and OVA-specific IgE than other groups. Oxidative stress as per the lipid peroxidation level in BALF of mGST-treated mice was reduced significantly in comparison to PBS- or GST-treated mice. In conclusion, inhalation of mGST reduced airway inflammation in mice. Mutated GST with reduced allergenicity has better therapeutic potential and can be explored as an adjunct therapy in asthma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 45, Issue 10, 15 November 2008, Pages 1413-1419
Journal: Free Radical Biology and Medicine - Volume 45, Issue 10, 15 November 2008, Pages 1413-1419
نویسندگان
Prabhanshu Tripathi, B.P. Singh, Naveen Arora,