کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1911681 1046828 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Degradation of glycated bovine serum albumin in microglial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Degradation of glycated bovine serum albumin in microglial cells
چکیده انگلیسی

Glycated protein products are formed upon binding of sugars to lysine and arginine residues and have been shown to accumulate during aging and in pathologies such as Alzheimer disease and diabetes. Often these glycated proteins are transformed into advanced glycation end products (AGEs) by a series of intramolecular rearrangements.In the study presented here we tested the ability of microglial cells to degrade BSA-AGE formed by glycation reactions of bovine serum albumin (BSA) with glucose and fructose. Microglial cells are able to degrade BSA-AGEs to a certain degree by proteasomal and lysosomal pathways. However, the proteasome and lysosomal proteases are severely inhibited by cross-linked BSA-AGEs. BSA-AGEs are furthermore able to activate microglial cells. This activation is accompanied by an enhanced degradation of BSA-AGE.Therefore, we conclude that microglial cells are able to degrade glycated proteins, although cross-linked protein–AGEs have an inhibitory effect on proteolytic systems in microglial cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 40, Issue 6, 15 March 2006, Pages 1017–1027
نویسندگان
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