کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911947 | 1046848 | 2006 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitric oxide modulates glutathione synthesis during endotoxemia
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کلمات کلیدی
LPScystathionaseAMGl-2-Oxothiazolidine-4-carboxylic acidGSHGAPDHRT-PCRNOSl-NAME - L-NAMENω-nitro-l-arginine methyl ester - N-Nitro-L-Arginine Methyl EsterAminoguanidine - آمینوگوئیدینAntioxidant enzymes - آنزیم های آنتی اکسیدانCysteine - سیستئینlipopolysaccharide - لیپوپلی ساکاریدγ-glutamylcysteine ligase - لیگاز γ-گلوتامیل سیستئینNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسGlutathione - گلوتاتیونglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Nitric oxide is known to modulate intracellular glutathione levels, but the relationship between nitric oxide synthesis and glutathione metabolism during endotoxemia is unknown. The present study was designed to examine the effects of increased nitric oxide formation on hepatic glutathione synthesis and antioxidant defense in endotoxemic mice. Our results demonstrate that hepatic glutathione synthesis is decreased for 24 h following injection of lipopolysaccharide (LPS). Administration of the cysteine precursor, L-2-oxothiazolidine-4-carboxylic acid (OTZ), failed to normalize hepatic glutathione concentration, and suggests that decreased γ-glutamylcysteine ligase activity is primarily responsible for the decrease in hepatic glutathione levels during endotoxemia. Inhibition of nitric oxide synthesis prevented the endotoxin-induced changes in hepatic and plasma glutathione status and up-regulated liver glutathione and cysteine synthesis pathways at the level of gene expression. Furthermore, whereas the activity of glutathione peroxidase and glutathione S-transferase decreased during endotoxemia, both of these changes were prevented by inhibition of nitric oxide synthesis. In conclusion, increased nitric oxide synthesis during endotoxemia causes marked changes in glutathione flux and defenses against oxidative stress in the liver.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 41, Issue 12, 15 December 2006, Pages 1817-1828
Journal: Free Radical Biology and Medicine - Volume 41, Issue 12, 15 December 2006, Pages 1817-1828
نویسندگان
Seyedmehdi Payabvash, Mohammad Hossein Ghahremani, Ardeshir Goliaei, Ali Mandegary, Hamed Shafaroodi, Massoud Amanlou, Ahmad Reza Dehpour,