کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1919185 1535609 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sphingolipid metabolism regulates development and lifespan in Caenorhabditis elegans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Sphingolipid metabolism regulates development and lifespan in Caenorhabditis elegans
چکیده انگلیسی


• Long-chain sphingolipids and ceramides accumulate in Caenorhabditis elegans during aging.
• Sphingolipid and ceramide levels are reduced in dauer and calorie-restricted worms.
• Pharmacological inhibition and genetic knockdown of SPT extends lifespan.
• Sphingolipid and ceramide metabolism regulate development rate and lifespan.

Sphingolipids are a highly conserved lipid component of cell membranes involved in the formation of lipid raft domains that house many of the receptors and cell-to-cell signaling factors involved in regulating cell division, maturation, and terminal differentiation. By measuring and manipulating sphingolipid metabolism using pharmacological and genetic tools in Caenorhabditis elegans, we provide evidence that the synthesis and remodeling of specific ceramides (e.g., dC18:1–C24:1), gangliosides (e.g., GM1–C24:1), and sphingomyelins (e.g., dC18:1–C18:1) influence development rate and lifespan. We found that the levels of fatty acid chain desaturation and elongation in many sphingolipid species increased during development and aging, with no such changes in developmentally-arrested dauer larvae or normal adults after food withdrawal (an anti-aging intervention). Pharmacological inhibitors and small interfering RNAs directed against serine palmitoyl transferase and glucosylceramide synthase acted to slow development rate, extend the reproductive period, and increase lifespan. In contrast, worms fed an egg yolk diet rich in sphingolipids exhibited accelerated development and reduced lifespan. Our findings demonstrate that sphingolipid accumulation and remodeling are critical events that determine development rate and lifespan in the nematode model, with both development rate and aging being accelerated by the synthesis of sphingomyelin, and its metabolism to ceramides and gangliosides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volumes 143–144, 15 December 2014, Pages 9–18
نویسندگان
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