کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1923246 1048882 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Redox-control of the alarmin, Interleukin-1α
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Redox-control of the alarmin, Interleukin-1α
چکیده انگلیسی


• Sod2-dependent increases in steady-state H2O2 promote IL-1α expression.
• H2O2 causes nuclear localization of IL-1α and extracellular Ca2+ influx.
• Inhibition of the Ca2+ regulated calpain prevents H2O2 dependent IL-1α nuclear localization.
• Nuclear IL-1α interacts with p300 and promotes NF-κB activity.

The pro-inflammatory cytokine Interleukin-1α (IL-1α) has recently emerged as a susceptibility marker for a wide array of inflammatory diseases associated with oxidative stress including Alzheimer's, arthritis, atherosclerosis, diabetes and cancer. In the present study, we establish that expression and nuclear localization of IL-1α are redox-dependent. Shifts in steady-state H2O2 concentrations (SS-[H2O2]) resulting from enforced expression of manganese superoxide dismutase (SOD2) drive IL-1α mRNA and protein expression. The redox-dependent expression of IL-1α is accompanied by its increased nuclear localization. Both IL-1α expression and its nuclear residency are abrogated by catalase co-expression. Sub-lethal doses of H2O2 also cause IL-1α nuclear localization. Mutagenesis revealed IL-1α nuclear localization does not involve oxidation of cysteines within its N terminal domain. Inhibition of the processing enzyme calpain prevents IL-1α nuclear localization even in the presence of H2O2. H2O2 treatment caused extracellular Ca2+ influx suggesting oxidants may influence calpain activity indirectly through extracellular Ca2+ mobilization. Functionally, as a result of its nuclear activity, IL-1α overexpression promotes NF-kB activity, but also interacts with the histone acetyl transferase (HAT) p300. Together, these findings demonstrate a mechanism by which oxidants impact inflammation through IL-1α and suggest that antioxidant-based therapies may prove useful in limiting inflammatory disease progression.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Redox Biology - Volume 1, Issue 1, 2013, Pages 218–225
نویسندگان
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