کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1925091 1536342 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death
ترجمه فارسی عنوان
هوموستازیس کلسیم متوقف شده است که در مرگ سلول های فورورسپتور ناشی از یون افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• ZnCl2 inhibits 661 W cell growth in time- and concentration-dependent manners.
• ZnCl2-treatment can induce the overgeneration of ROS within 661 W cells.
• ZnCl2 exposure causes cell cycle arrest at G2/M phases in 661 W cells.
• ZnCl2 elevates the cytosolic [Ca2+] and disrupts the calcium homeostasis.
• ZnCl2 suppresses PMCA-1 and PMCA-2 expression and their activities.

Zinc (Zn), the second abundant trace element in living organisms, plays an important role in regulating cell metabolism, signaling, proliferation, gene expression and apoptosis. Meanwhile, the overload of Zn will disrupt the intracellular calcium homeostasis via impairing mitochondrial function. However, the specific molecular mechanism underlying zinc-induced calcium regulation remains poorly understood. In the present study, using zinc chloride (ZnCl2) as a stressor, we investigated the effect of exogenous Zn2+ in regulating murine photoreceptor cell viability, reactive oxygen species (ROS), cell cycle distribution and calcium homeostasis as well as plasma membrane calcium ATPase (PMCA) isoforms (PMCA1 and PMCA2, i.e., ATP2B1, ATP2B2) expression. We found that the exogenous Zn2+ in the exposure range (31.25–125.0 μmol/L) results in the overgeneration of ROS, cell cycle arrest at G2/M phases, elevation of cytosolic [Ca2+], inactivation of Ca2+-ATPase and reduction of both PMCA1 and PMCA2 in 661 W cells, and thus induces cell death. In conclusion, ZnCl2 exposure can elevate the cytosolic [Ca2+], disrupt the intracellular calcium homeostasis, further initiate Ca2+-dependent signaling pathway in 661 W cells, and finally cause cell death. Our results will facilitate the understanding of cell death induced by the zinc ion-mediated calcium homeostasis disruption.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 560, 15 October 2014, Pages 44–51
نویسندگان
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