کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1925438 | 1536376 | 2013 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Antigen-presenting cells in parotid glands contain cystatin D originating from acinar cells Antigen-presenting cells in parotid glands contain cystatin D originating from acinar cells](/preview/png/1925438.png)
Cystatin D encoded by Cst5 is a salivary classified type II cystatin. We investigated the dynamism of cystatin D by examining the distribution of cystatin D protein and mRNA in rats, to identify novel functions. The simultaneous expression of Cst5 and cystatin D was observed in parotid glands, however in situ hybridization showed that only acinar cells produced cystatin D. Synthesized cystatin D was localized in small vesicles and secreted from the apical side to the saliva, and from the basolateral side to the extracellular region, a second secretory pathway for cystatin D. We also identified antigen-presenting cells in the parotid glands that contained cystatin D without the expression of Cst5, indicating the uptake of cystatin D from the extracellular region. Cystatin D was detected in blood serum and renal tubular cells with megalin, indicating the circulation of cystatin D through the body and uptake by renal tubular cells. Thus, the novel dynamism of cystatin D was shown and a function for cystatin D in the regulation of antigen-presenting cell activity was proposed.
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► Dynamism of salivary cystatin D, in blood, antigen presenting cells, and kidney.
► Cystatin D is produced in parotid acinar cells and localized in small vesicles.
► Cystatin D and megalin is colocalized in renal tubule cells.
► Cystatin D would affect regulation of antigen presenting cells.
Journal: Archives of Biochemistry and Biophysics - Volume 530, Issue 1, 1 February 2013, Pages 32–39