Conversion of pro-inflammatory murine Alox5 into an anti-inflammatory 15S-lipoxygenating enzyme by multiple mutations of sequence determinants

► Reaction specificity of lipoxygenases (LOX) is crucial for their biological roles. ► 5-LOX acts pro-inflammatory but 15-LOX exhibits anti-inflammatory properties. ► 5-LOX can be converted to 15-LOX by site directed mutagenesis. ► Reduction of the active site volume is discussed as basis for this alteration.