کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1925573 1536393 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of zinc in catalytic activity of carbonic anhydrase IX
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Role of zinc in catalytic activity of carbonic anhydrase IX
چکیده انگلیسی

The carbonic anhydrases (CAs) in the α class are zinc-dependent metalloenzymes. Previous studies have reported that recombinant forms of carbonic anhydrase IX (CAIX), a membrane-bound form of CA expressed in solid tumors, appear to be activated by low levels of zinc independent of its well-studied role at the catalytic site. In this study, we sought to determine if CAIX is stimulated by zinc in its native environment. MDA-MB-231 breast cancer cells express CAIX in response to hypoxia. We compared CAIX activity associated with membrane ghosts isolated from hypoxic cells with that in intact hypoxic cells. We measured CA activity directly using 18O exchange from 13CO2 into water determined by membrane inlet mass spectrometry. In membrane ghosts, there was little effect of zinc at low concentrations on CAIX activity, although at high concentration zinc was inhibitory. In intact cells, zinc had no significant effect on CAIX activity. This suggests that there is an appreciable decrease in sensitivity to zinc when CAIX is in its natural membrane milieu compared to the purified forms.


► CAIX activity in membranes is not affected by zinc at physiological concentrations.
► CAIX activity associated with hypoxic MDA-MB-231 cells is not affected by zinc.
► Data contrast to recombinant forms of CAIX which are activated by zinc.
► Natural membrane milieu reduces sensitivity of CAIX to zinc.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 521, Issues 1–2, May 2012, Pages 90–94
نویسندگان
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