کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1926390 | 1536463 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional role of the charge at the T538 residue in the control of protein kinase Cθ
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Functional role of the charge at the T538 residue in the control of protein kinase Cθ Functional role of the charge at the T538 residue in the control of protein kinase Cθ](/preview/png/1926390.png)
چکیده انگلیسی
We show that protein kinase C (PKC) θ localized at the Golgi complex is partially conjugated to monoubiquitin. Using the inactive T538A and activable T538E mutants of PKCθ, we demonstrate that the presence of an uncharged residue at the 538 position of the activation loop favors both association with the Golgi and monoubiquitination of the kinase. Moreover, the inactive PKCθ does not translocate from the Golgi in response to a short-term cell stimulation with a phorbol ester and is subjected to different proteolytic degradation pathways compared to the activable cytosolic kinase. These findings highlight the role of T538 as a critical determinant to address the activable and the inactive PKCθ molecules to different intracellular compartments and to specific post-transductional modifications. The functional relevance of these observations is supported by the impaired cell division observed in phenotypes expressing high levels of the inactive PKCθ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 481, Issue 2, 15 January 2009, Pages 202-209
Journal: Archives of Biochemistry and Biophysics - Volume 481, Issue 2, 15 January 2009, Pages 202-209
نویسندگان
Mario Passalacqua, Marco Pedrazzi, Bianca Sparatore, Mauro Patrone, Sandro Pontremoli, Edon Melloni,