NS5ATP9 gene regulated by NF-κB signal pathway

NS5ATP9 was previously identified as p15PAF [proliferating cell nuclear antigen (PCNA)-associated factor] to bind with PCNA. We earlier identified the promoter region of NS5ATP9 and found NS5ATP9 is a NS5A up-regulation gene. However little is known about how it is regulated. To investigate the gene regulation of NS5ATP9, we screened NS5ATP9 promoter binding proteins using phage display and verified by electrophoretic mobility shift assay (EMSA). We found that the nuclear protein rhNF-κB (p50) could bind to the NS5ATP9 promoter and the binding region contained within a 156 bp (nucleotides −5 to −161 bp) immediately upstream of the transcription initiation site. Our results suggest that NF-κB could participate in the regulation of NS5ATP9 gene expression in carcinogenesis.