کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1926921 1536500 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spectrally and spatially resolved fluorescence lifetime imaging in living cells: TRPV4–microfilament interactions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Spectrally and spatially resolved fluorescence lifetime imaging in living cells: TRPV4–microfilament interactions
چکیده انگلیسی

Time- and space-correlated single photon counting method has been used to demonstrate the interactions of cation channel “transient receptor potential vanilloid 4” (TRPV4) and microfilaments. Living cells co-expressing TRPV4-CFP and actin-YFP, when excited for the donor molecules (CFP) exhibited an emission peak at 527 nm and decrease of the lifetime in the wavelength band 460–490 nm; corresponding to resonance energy transfer to YFP. CFP fluorescence decay was fitted best by a dual mode decay model. Considering the average lifetime of the donor, both in the presence and absence of acceptor yielded an apparent FRET efficiency of ∼20%. This is rather high placing the minimum distance of chromophores in the two fluorescent proteins in the range of 4 nm. Thus, this study shows for the first time that TRPV4 and actin intimately associate within living cells. The significance of this finding for cell volume regulation is highlighted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 463, Issue 1, 1 July 2007, Pages 27–36
نویسندگان
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