کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1927021 | 1536494 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overexpression of cardiac actin with baculovirus is promoter dependent
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Overexpression of cardiac actin with baculovirus is promoter dependent Overexpression of cardiac actin with baculovirus is promoter dependent](/preview/png/1927021.png)
چکیده انگلیسی
The influence of the promoter and an N-terminal hexahistidine tag on human cardiac actin (ACTC) expression and function was investigated using four baculovirus constructs. It was found that both non-tagged ACTC and hisACTC expression from the p10 promoter was higher than from the polh promoter. Characterization showed that an N-terminal hexahistidine tag has a negative effect on ACTC. Recombinant ACTC inhibits DNase-I and binds myosin S1, indicative of proper folding. Our data support the hypothesis that the actin protein down-regulates the polh promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 466, Issue 1, 1 October 2007, Pages 58–65
Journal: Archives of Biochemistry and Biophysics - Volume 466, Issue 1, 1 October 2007, Pages 58–65
نویسندگان
Susan P. Yates, Marissa D. Otley, John F. Dawson,