کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1927318 1536508 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of Clara cell secretory protein gene expression by the CCAAT-binding factor NF-Y
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Regulation of Clara cell secretory protein gene expression by the CCAAT-binding factor NF-Y
چکیده انگلیسی

Analysis of the transcriptional regulation of the Clara cell secretory protein (CCSP) gene has resulted in the characterization of several trans-acting factors that regulate the activity of this gene. However, little is known about negative regulatory elements involved in CCSP gene transcription. Using transient transfections of luciferase reporter constructs driven by various fragments of the Neotomodon CCSP (nCCSP) promoter, we identified an inhibitory region that contains an inverted CCAAT box located −225 to −221 bp upstream of the transcriptional start site. Sequence analysis in a broad region of the nCCSP promoter (−744/+33) identified another potentially important CCAAT motif (−459/−455). Gel shift and supershift assays indicated that the transcription factor NF-Y binds to both CCAAT boxes. Mutation of the CCAAT motif prevented the in vitro binding of NF-Y and led to a significant increase of CCSP promoter activity in both pulmonary (H441) and non-pulmonary (HeLa and MCF-7) cells, suggesting that NF-Y is involved in a negative transcriptional regulation that may potentially contribute to the highly cell-specific expression of the anti-inflammatory CCSP gene.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 459, Issue 1, 1 March 2007, Pages 33–39
نویسندگان
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