کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1942115 1052576 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of subunit III and its lipids in the molecular mechanism of cytochrome c oxidase
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Role of subunit III and its lipids in the molecular mechanism of cytochrome c oxidase
چکیده انگلیسی


• Atomistic molecular dynamics simulations on cytochrome c oxidase are performed.
• Lipids in subunit III influence the rate of proton transfer through D-pathway.
• The absence of subunit III-bound lipids may affect the O2 diffusion towards active-site.

The terminal respiratory enzyme cytochrome c oxidase (CcO) reduces molecular oxygen to water, and pumps protons across the inner mitochondrial membrane, or the plasma membrane of bacteria. A two-subunit CcO harbors all the elements necessary for oxygen reduction and proton pumping. However, it rapidly undergoes turnover-induced irreversible damage, which is effectively prevented by the presence of subunit III and its tightly bound lipids. We have performed classical atomistic molecular dynamics (MD) simulations on a three-subunit CcO, which show the formation of water wires between the polar head groups of lipid molecules bound to subunit III and the proton uptake site Asp91 (Bos taurus enzyme numbering). Continuum electrostatic calculations suggest that these lipids directly influence the proton affinity of Asp91 by 1–2 pK units. We surmise that lipids bound to subunit III influence the rate of proton uptake through the D-pathway, and therefore play a key role in preventing turnover-induced inactivation. Atomistic MD simulations show that subunit III is rapidly hydrated in the absence of internally bound lipids, which is likely to affect the rate of O2 diffusion into the active-site. The role of subunit III with its indigenous lipids in the molecular mechanism of CcO is discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issue 8, August 2015, Pages 690–697
نویسندگان
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