کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1942370 | 1052609 | 2012 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Factors determining the fluorescence yield of fucoxanthin-chlorophyll complexes (FCP) involved in non-photochemical quenching in diatoms Factors determining the fluorescence yield of fucoxanthin-chlorophyll complexes (FCP) involved in non-photochemical quenching in diatoms](/preview/png/1942370.png)
Fucoxanthin-chlorophyll complexes (FCP) from the centric diatom Cyclotella meneghiniana were isolated and the trimeric FCPa complex was reconstituted into liposomes at different lipid to Chl a ratios. The fluorescence yield of the complexes in different environments was calculated from room temperature fluorescence emission spectra and compared to the aggregated state of FCPa. FCPa surrounded by high amounts of lipids resembled detergent solubilised complexes and with decreasing lipid levels, i.e. in a situation where protein contacts were increasingly favoured, the fluorescence yield of FCPa gradually decreased. In addition, the yield displayed a strong pH-dependency in case of lower lipid contents. The further reduction in fluorescence yield brought about by the conversion of diadinoxanthin to diatoxanthin was pH independent and only depended on the amount of diatoxanthin synthesised. The implications of these data for non-photochemical quenching in centric diatoms are discussed.
► Fluorescence yield of FCPa depends on protein-protein interactions in lipids.
► Lower pH decreases the yield the more the lower the amount of lipids present.
► pH sensitive amino acid residues are found in both constitutive polypeptides.
► Diatoxanthin acts independently of the pH.
► Diatoxanthin acts in addition to the effects induced by protein interaction.
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1817, Issue 7, July 2012, Pages 1044–1052