کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1942669 1052623 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice
چکیده انگلیسی

Mitochondrial uncoupling protein 3 (UCP3) is constitutively expressed in mitochondria from thymus and spleen of mice, and confocal microscopy has been used to visualize UCP3 in situ in mouse thymocytes. UCP3 is present in mitochondria of thymus and spleen up to at least 16weeks after birth, but levels decrease by a half in thymus and a fifth in spleen after three weeks, probably reflecting the suckling to weaning transition. UCP3 protein levels increase ~ 3-fold in thymus on starvation, but expression levels in spleen were unaffected by starvation. Lack of UCP3 had little effect on thymus mass or thymocyte number. However, lack of UCP3 affected spleen mass and splenocyte number (in the fasted state) and results in reduced CD4+ single positive cell numbers and reduced double negative cells in the thymus, but as a 2-fold increase in the proportion of CD4+, CD8+ and DP cells in spleen. Starvation attenuates these proportionate differences in the spleen. A lack of UCP3 had no apparent effect on basal oxygen consumption of thymocytes or splenocytes or on oxygen consumption due to mitochondrial proton leak. Splenocytes from UCP3 knock-out mice are also more resistant to apoptosis than those from wild-type mice. Overall we can conclude that UCP3 affects thymocyte and spleen cell profiles in the fed and fasted states.

Research Highlights
► UCP3 present in thymus and spleen.
► No effect of absence of UCP3 on thymus mass and thymocyte number.
► Absence of UCP3 affected spleen mass and splenocytes number in fasted state.
► Lack of UCP3 increases CD4 and CD8 single positive T-cells in the spleen.
► Lack of UCP3 makes splenocytes more resistant to apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1807, Issue 9, September 2011, Pages 1064–1074
نویسندگان
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