کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1943035 1052642 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurements of mitochondrial calcium in vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Measurements of mitochondrial calcium in vivo
چکیده انگلیسی

Mitochondria play a pivotal role in intracellular Ca2+ signalling by taking up and releasing the ion upon specific conditions. In order to do so, mitochondria depend on a number of factors, such as the mitochondrial membrane potential and spatio-temporal constraints. Whereas most of the basic principles underlying mitochondrial Ca2+ handling have been successfully deciphered over the last 50 years using assays based on in vitro preparations of mitochondria or cultured cells, we have only just started to understand the actual physiological relevance of these processes in the whole animal. Recent advancements in imaging and genetically encoded sensor technologies have allowed us to visualise mitochondrial Ca2+ transients in live mice. These studies used either two-photon microscopy or bioluminescence imaging of cameleon or aequorin-GFP Ca2+ sensors, respectively. Both methods revealed a consistent picture of Ca2+ uptake into mitochondria under physiological conditions even during very short-lasting elevations of cytosolic Ca2+ levels. The big future challenge is to understand the functional impact of such Ca2+ signals on the physiology of the observed tissue as well as of the whole organism. To that end, the development of multiparametric in vivo approaches will be mandatory.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1787, Issue 11, November 2009, Pages 1317–1323
نویسندگان
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