کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944115 1053179 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heat-activated liposome targeting to streptavidin-coated surfaces
ترجمه فارسی عنوان
لیپوزوم با فعالیت گرم با هدف قرار دادن سطوح پوشیده شده با استرپتایدین
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Asymmetric liposomes were prepared by phospholipase hydrolysis symmetric liposomes.
• The asymmetry of the prepared liposomes was well preserved in the gel phase.
• Binding of the asymmetric liposomes to streptavidin was activated during heating.

There is a great need of improved anticancer drugs and corresponding drug carriers. In particular, liposomal drug carriers with heat-activated release and targeting functions are being developed for combined hyperthermia and chemotherapy treatments of tumors. The aim of this study is to demonstrate the heat-activation of liposome targeting to biotinylated surfaces, in model experiments where streptavidin is used as a pretargeting protein. The design of the heat-activated liposomes is based on liposomes assembled in an asymmetric structure and with a defined phase transition temperature. Asymmetry between the inside and the outside of the liposome membrane was generated through the enzymatic action of phospholipase D, where lipid head groups in the outer membrane leaflet, i.e. exposed to the enzyme, were hydrolyzed. The enzymatically treated and purified liposomes did not bind to streptavidin-modified surfaces. When activation heat was applied, starting from 22 °C, binding of the liposomes occurred once the temperature approached 33 ± 0.5 °C. Moreover, it was observed that the asymmetric structure remained stable for at least 2 weeks. These results show the potential of asymmetric liposomes for the targeted binding to cell membranes in response to (external) temperature stimulus. By using pretargeting proteins, this approach can be further developed for personalized medicine, where tumor-specific antibodies can be selected for the conjugation of pretargeting agents.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1848, Issue 6, June 2015, Pages 1417–1423
نویسندگان
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