کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944377 1053210 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine
چکیده انگلیسی

Gene therapy is expected to treat various incurable diseases including viral infections, autoimmune disorders, and cancers. Cationic lipids (CL) have been used as carriers of therapeutic DNAs for gene therapy because they can form a complex with DNA and such a complex can be incorporated into cells and transport the bound DNA to cytosol. The CL/DNA complexes are called lipoplexes and categorized as a non-viral vector. Lipoplexes are often prepared by adding a neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) to CL in order to enhance transfection. However, the role of DOPE is not fully understood. We synthesized a new CL having an ethylenediamine cationic head group, denoted by DA, and found that addition of DOPE to DA achieved a good efficiency, almost in the similar level of commonly used transfection reagent Lipofectamine 2000 (Invitrogen). The composition of DA:DOPE = 1:1 showed the highest efficiency. This lipoplex showed structural transition when pH was changed from 7 to 4, corresponding pH lowering in late endosome, while DOPE itself showed structural transition at more basic pH around 8. The present data showed that the DOPE/DA composition determines the structural transition pH and choosing a suitable pH, i.e., a suitable composition, is essential to increase the transfection efficiency.

Addition of a neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) to cationic lipid (DA) can achieve a better transfection efficiency than commonly used transfection reagent.Figure optionsDownload high-quality image (137 K)Download as PowerPoint slideHighlights
► We synthesized a new cationic lipid having an ethylenediamine head group (DA).
► We found that the addition of DOPE to DA achieved a better efficiency.
► DOPE is responsible for the pH responsive structural transition.
► The adding of DA can tune the transition pH well-suited to the pH change.
► This role of DOPE can be generalized for other cationic lipids containing DOPE.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1828, Issue 2, February 2013, Pages 412–418
نویسندگان
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