کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944462 1053211 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Arabidopsis KEA2, a homolog of bacterial KefC, encodes a K+/H+ antiporter with a chloroplast transit peptide
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Arabidopsis KEA2, a homolog of bacterial KefC, encodes a K+/H+ antiporter with a chloroplast transit peptide
چکیده انگلیسی

KEA genes encode putative K+ efflux antiporters that are predominantly found in algae and plants but are rare in metazoa; however, nothing is known about their functions in eukaryotic cells. Plant KEA proteins show homology to bacterial K+ efflux (Kef) transporters, though two members in the Arabidopsis thaliana family, AtKEA1 and AtKEA2, have acquired an extra hydrophilic domain of over 500 residues at the amino terminus. We show that AtKEA2 is highly expressed in leaves, stems and flowers, but not in roots, and that an N-terminal peptide of the protein is targeted to chloroplasts in Arabidopsis cotyledons. The full-length AtKEA2 protein was inactive when expressed in yeast; however, a truncated AtKEA2 protein (AtsKEA2) lacking the N-terminal domain complemented disruption of the Na+(K+)/H+ antiporter Nhx1p to confer hygromycin resistance and tolerance to Na+ or K+ stress. To test transport activity, purified truncated AtKEA2 was reconstituted in proteoliposomes containing the fluorescent probe pyranine. Monovalent cations reduced an imposed pH gradient (acid inside) indicating AtsKEA2 mediated cation/H+ exchange with preference for K+ = Cs+ > Li+ > Na+. When a conserved Asp721 in transmembrane helix 6 that aligns to the cation binding Asp164 of Escherichia coli NhaA was replaced with Ala, AtsKEA2 was completely inactivated. Mutation of a Glu835 between transmembrane helix 8 and 9 in AtsKEA2 also resulted in loss of activity suggesting this region has a regulatory role. Thus, AtKEA2 represents the founding member of a novel group of eukaryote K+/H+ antiporters that modulate monovalent cation and pH homeostasis in plant chloroplasts or plastids.


► Plant KEA proteins share high homology to bacterial KefC/B antiporters.
► Full‐length AtKEA2 is inactive in yeast.
► AtKEA2 without N-terminal domain complements yeast Nhx1p.
► Purified and reconstituted short AtKEA2 protein has K+/H+ exchange activity.
► Cation specificity of AtKEA2 is similar to EcKefC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1818, Issue 9, September 2012, Pages 2362–2371
نویسندگان
, , , , , ,