The role of the C-terminal region of pulchellin A-chain in the interaction with membrane model systems

► We investigated the interaction between recombinant PAC and Langmuir monolayers. ► Three catalytically active, truncated PACs with increasing deletion of the C-terminal region were studied. ► The affinity to DPPG decreased with increased deletion of the C-terminal region. ► Using PM-IRRAS we confirm the importance of the C-terminal region for PAC-membrane interaction. ► We consider this interaction fundamental for the protein to reach the cytosol and develop its toxic activity.