کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944616 1053231 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of liposomes’ surface electric potential on the uptake of hematoporphyrin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The effect of liposomes’ surface electric potential on the uptake of hematoporphyrin
چکیده انگلیسی

Hematoporphyrin is being used as a photosensitizer in photodynamic therapy of tumors, as well as of other clinical cases. Many classes of tetrapyrroles, including hematoporphyrin, are partitioning quite easily into the external cytoplasmic membrane as the mechanism of cellular uptake. Several chemical and physical parameters of the membrane were studied for their effect on the extent of porphyrins' partitioning. In this manuscript we report, for the first time, a quantitative analysis of the effect of the membrane's surface electric potential on the partitioning. We prepared liposomes, as membrane models, composed on zwitterionic DMPC lipid, as well as DMPC liposomes that contain a small, varying fraction of negatively charged DMPS and positively charged DOTAP. We found that indeed the surface potential had a very strong effect on the binding constant of HP, which is negatively charged at the physiological pH that was used. The trend in the apparent binding constant can be formulated and fitted with the Gouy-Chapman model of surface potential. We found that the average concentration of HP within the aqueous shell that has a thickness of the Debye layer around the liposome is determining the extent of binding in the law of mass action.

Research Highlights
► We probed the effect of membrane electric surface potential on the binding of hematoporphyrin to liposomes.
► The binding is very strongly affected by the surface potential, which was generated by the addition of charged lipid molecules.
► The trend in the binding constant can be nicely accommodated with the Gouy-Chapman model of surface potential.
► The local average concentration of hematoporphyrin in the Debye layer around the liposome is the factor which determines the equilibrium of binding.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1808, Issue 8, August 2011, Pages 2031–2035
نویسندگان
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