کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944886 1537145 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of the channel-forming domain of Clostridium perfringens Epsilon-toxin (ETX)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of the channel-forming domain of Clostridium perfringens Epsilon-toxin (ETX)
چکیده انگلیسی

Epsilon-toxin (ETX) is a potent toxin produced by Clostridium perfringens strains B and D. The bacteria are important pathogens in domestic animals and cause edema mediated by ETX. This toxin acts most likely by heptamer formation and rapid permeabilization of target cell membranes for monovalent anions and cations followed by a later entry of calcium. In this study, we compared the primary structure of ETX with that of the channel-forming stretches of a variety of binding components of A-B-types of toxins such as Anthrax protective antigen (PA), C2II of C2-toxin and Ib of Iota-toxin and found a remarkable homology to amino acids 151–180 of ETX. Site-directed mutagenesis of amino acids within the putative channel-forming domain resulted in changes of cytotoxicity and effects on channel characteristics in lipid bilayer experiments including changes of selectivity and partial channel block by methanethiosulfonate (MTS) reagents and antibodies against His6-tags from the trans-side of the lipid bilayer membranes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1788, Issue 12, December 2009, Pages 2584–2593
نویسندگان
, , , , ,