کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1945126 1537146 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hemoglobin precipitation by polyethylene glycols leads to underestimation of membrane pore sizes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Hemoglobin precipitation by polyethylene glycols leads to underestimation of membrane pore sizes
چکیده انگلیسی

The size of pores formed in the plasma membrane by various substances is frequently determined using polyethylene glycols as osmotic protectants. In this work, we have found that the size of pores formed by saponin in the red blood cell membrane determined by hemolysis versus molecular weight of polyethylene glycol was different to that estimated by light dispersion of cell suspensions. After complete swelling of cells induced by saponin in semiisotonic salt media containing 150 mOsm PEG-4000 or PEG-3000, a significant increase in the light absorbance at 640 nm was developed resulting from the formation of hemoglobin precipitates. Easily sedimenting aggregates were also formed when the supernatant of lysed cells was added to the equiosmotic solutions of polyethylene glycols with molecular weight higher than 1000. We suggest that the real size of large pores could be underestimated due to the phenomenon of hemoglobin precipitation by polyethylene glycols.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1778, Issue 12, December 2008, Pages 2775–2780
نویسندگان
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