کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1945498 1053266 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrogenic steps of the SR Ca-ATPase enzymatic cycle and the effect of curcumin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Electrogenic steps of the SR Ca-ATPase enzymatic cycle and the effect of curcumin
چکیده انگلیسی
Sarcoplasmic reticulum (SR) vesicles were adsorbed on an octadecanethiol/phosphatidylcholine mixed bilayer anchored to a gold electrode, and the Ca-ATPase contained in the vesicles was activated by ATP concentration jumps in the presence of calcium ions. The resulting capacitive current transients are compared with those calculated on the basis of the enzymatic cycle of the calcium pump. This comparison provides information on the kinetics of the E2-E1 conformational change and on its pH dependence. The alteration in the current transients following ATP concentration jumps in the presence of curcumin is examined. In particular, curcumin decreases the rate of slippage of the Ca-ATPase, and at concentrations above 10 μM reduces calcium transport by this pump.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1778, Issue 2, February 2008, Pages 405-413
نویسندگان
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