کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1945837 | 1053278 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of liposomes coated with S-layer proteins from lactobacilli Characterization of liposomes coated with S-layer proteins from lactobacilli](/preview/png/1945837.png)
The stability of liposomes coated with S-layer proteins from Lactobacillus brevis and Lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. The interactions of the different S-layer proteins with positively charged liposomes prepared with soybean lecithin or dipalmitoylphosphatidylcholine were studied by means of the variation of the Z potential at different protein–lipid ratios, showing that both proteins were able to attach in a greater extent to the surface of soybean lecithin liposomes. The capacity of these particles to retain carboxyfluorescein or calcein by exposure to bile salts, pancreatic extract, pH change and after a thermal shock showed that both S-layer proteins increased the stability of the liposomes in the same magnitude. The non-glycosylated protein from L. brevis protects more efficiently the liposomes at pH 7 than those from L. kefir even without treatment with glutaraldehyde.
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1768, Issue 3, March 2007, Pages 393–400