کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1946779 | 1054270 | 2010 | 6 صفحه PDF | دانلود رایگان |
PAI-1 (plasminogen activator inhibitor-1) in breast cancer cells is involved in tumour development and metastasis of breast cancer cells. PAI-1 function and the regulation of its expression have been precisely investigated. Here we report that EGF, which promotes breast cancer tumour growth and survival, rapidly induces PAI-1 expression in the breast adenocarcinoma cell line MCF-7 through the activation of the transcription factor Elk-1.We have found that the PAI-1 promoter fragment (−140 to + 173) containing the Ets consensus binding site is activated by Elk-1. Chromatin immunoprecipitation analysis confirms in vivo binding of Elk-1 to the PAI-1 promoter and demonstrates that Elk-1 phosphorylation on the Ets binding site is EGF-dependent.
Research highlights
► EGF activates ERK.
► Activation of transcription factor Elk-1.
► Regulation of human PAI-1 expression via the sequence TTCCTG (CAGGAA on the complementary strand) located between −42 and −36bp. Elk-1 is bound directly to this sequence in the absence of SRF.
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1799, Issue 9, September 2010, Pages 616–621