کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1947094 | 1537318 | 2007 | 6 صفحه PDF | دانلود رایگان |
A rice diterpene cyclase, OsDTC2, functions as a stemar-13-ene synthase that converts syn-copalyl diphosphate into stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The transcriptional expression of OsDTC2 is induced by treatment of suspension-cultured rice cells with a chitin oligosaccharide elicitor. To investigate the molecular mechanisms of the elicitor signaling pathway that leads to OsDTC2 expression, we carried out deletion and mutation analysis of the region − 1939 bp upstream of the transcription start site of OsDTC2 in rice cells using dual luciferase assays. The region between − 1709 and − 1450 bp was found to contain six W-box motifs, which are putative recognition sites for WRKY transcription factors, as cis elements involved in elicitor-responsiveness and/or basic promoter activity of OsDTC2.
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1769, Issues 11–12, November–December 2007, Pages 678–683