کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1947138 | 1537319 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Translational regulation of human methionine synthase by upstream open reading frames
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کلمات کلیدی
ORFIRESuORFGene regulation - تنظیم ژنinternal ribosome entry site - سایت ورودی ریبوزوم داخلیupstream open reading frame - فریم خواندن باز بارگذاری بالادستopen reading frame - قاب خواندن بازMethionine - متیونینMethionine synthase - متیونین سنتازUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Translational regulation of human methionine synthase by upstream open reading frames Translational regulation of human methionine synthase by upstream open reading frames](/preview/png/1947138.png)
چکیده انگلیسی
Methionine synthase is a key enzyme poised at the intersection of folate and sulfur metabolism and functions to reclaim homocysteine to the methionine cycle. The 5â² leader sequence in human MS is 394 nucleotides long and harbors two open reading frames (uORFs). In this study, regulation of the main open reading frame by the uORFs has been elucidated. Both uORFs downregulate translation as demonstrated by mutation of the upstream AUG codons (uAUG) either singly or simultaneously. The uAUGs are capable of recruiting the 40S ribosomal complex as revealed by their ability to drive reporter expression in constructs in which the luciferase is fused to the uORFs. uORF2, which is predicted to encode a 30 amino acid long polypeptide, has a clustering of rare codons encoding arginine and proline. Mutation of a tandemly repeated rare codon for arginine at positions 3 and 4 in uORF2 to either common codons for the same amino acid or common codons for alanine results in complete alleviation of translation inhibition. This suggests a mechanism for ribosome stalling and demonstrates that the cis-effects on translation by uORF2 is dependent on the nucleotide sequence but is apparently independent of the sequence of the encoded peptide. This study reveals complex regulation of the essential housekeeping gene, methionine synthase, by the uORFs in its leader sequence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1769, Issues 9â10, SeptemberâOctober 2007, Pages 532-540
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1769, Issues 9â10, SeptemberâOctober 2007, Pages 532-540
نویسندگان
Bekir Col, Sebastian Oltean, Ruma Banerjee,