کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1947223 1054305 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of human cytoglobin gene promoter region
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Characterization of human cytoglobin gene promoter region
چکیده انگلیسی
Cytoglobin (CYGB) is a member of the vertebrate globin family together with hemoglobin, myoglobin and neuroglobin. Although the physiological function of CYGB is still unclear, spectroscopic studies show that CYGB contains a hexacoordinated heme pocket similar to other pentacoordinated globin proteins. CYGB shares a common phylogenetic ancestry with vertebrate myoglobin from which it diverged by duplication before the appearance of jawed vertebrates. The objective of this study is to identify the regulatory and promoter region of the human cytoglobin gene. 5′ unidirectional deletion constructs demonstrated that the proximal promoter elements of human CYGB gene are located between −1113 to −10 relative to the translation start site. Site-directed mutagenesis showed that mutation of a c-Ets-1 motif at −1008 and Sp1 motifs at −400, −230 and −210 remarkably decreased the promoter activity. Gel shift assays confirmed the binding of DNA-nuclear proteins to these motifs. All these results indicate that CYGB gene expression can be up-regulated by c-Ets-1 and Sp1 motifs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1759, Issue 5, May 2006, Pages 208-215
نویسندگان
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