کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1947225 | 1054305 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain
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کلمات کلیدی
PDICBBALRErv1Coomassie Brilliant Blue - Coomassie درخشان آبیExpression pattern - الگوی بیانAugmenter of liver regeneration - تقویت کننده بازسازی کبدAlternative splicing - جابجایی جایگزینGenomic organization - سازمان ژنومیMALDI-TOF MS - مالدی توف MSprotein disulfide isomerase - پروتئین دیسولفید ایزومراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Flavoproteins of the quiescin/sulfhydryl oxidase (QSOX) family catalyze oxidation of peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. We report here the molecular cloning of a new putative sulfhydryl oxidase cDNA, rQSOX-L (GenBank Accession no AY623665), from adult rat brain and its expression studied by RT-PCR, Northern and Western blots in rat tissues. DNA-sequencing demonstrated the existence of two cDNAs in rat cortex, corresponding to a long transcript (rQSOX-L) and a short transcript (rQSOX-S) which differed by 851 nucleotides due to alternative splicing. The new transcript, rQSOX-L (3356 nucleotides), was specifically expressed in brain, hypophysis, heart, testis and seminal vesicle. The distribution of this variant is not homogeneous in the different tissues studied and suggests a complex gene regulation. The full-length rQSOX-L cDNA has an open reading frame of 2250-bp encoding a protein of 750 amino acids that contains a signal peptide sequence, a protein-disulfide-isomerase-type thioredoxin and ERV1-ALR domains and a long form specific C-terminal extension. The rQSOX-L protein is highly homologous to members of the sulfhydryl oxidase/Quiescin family and contains particularly two potential sites for N-glycosylation. This protein isoform was specifically detected in rat brain tissues in opposition to the low molecular form that was ubiquitous. Matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis of the immunoprecipitate tryptic fragments allowed the identification of rQSOX-L protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1759, Issue 5, May 2006, Pages 225-233
Journal: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression - Volume 1759, Issue 5, May 2006, Pages 225-233
نویسندگان
Jean Radom, Didier Colin, Franck Thiebault, Mai Dognin-Bergeret, Georges Mairet-Coello, Annick Esnard-Feve, Dominique Fellmann, Michèle Jouvenot,