Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis

► Branching enzyme of R. obamensis (RoBE) requires minimal DP10 donor glucans. ► RoBE produces highly branched polymers from a mix of polydisperse linear glucans. ► The branching process is preferably intermolecular. ► Branched polymers are preferred acceptor substrates. ► RoBE has an extra α-4-glucanotransferase activity not reported yet for such enzyme.