Endogenous Bmp4 in myoblasts is required for myotube formation in C2C12 cells

► Expression of Bmp4 is increased with progression of myogenesis. ► Proliferating myoblasts express Bmp4. ► Rgm c, a co-receptor to enhance Bmp signaling, is predominantly expressed in myotubes. ► Down-regulation of Bmp signal components inhibits myotube differentiation/maturation. ► Transcription of Bmp4 is repressed by the presence of myotubes.