Characterization of the in vitro binding and inhibition kinetics of primary amine oxidase/vascular adhesion protein-1 by glucosamine

► Primary-amine oxidase mediates the adhesion of lymphocytes during inflammation. ► Glucosamine is widely used as a supplement for the treatment of arthritis. ► We show glucosamine to be a time-dependent inhibitor of PrAO activity. ► In vitro inhibition and binding were augmented in the presence of H2O2. ► Limitation: millimolar glucosamine concentrations required for inhibition in vitro.