کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1948584 | 1054702 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Self-complementary sequences induce the formation of double-stranded filamentous phages
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The single strand nature of the filamentous phage (Ff) genome is currently one of the main drawbacks for their application as gene delivery vectors. In this work, by the incorporation of inverted self complementary sequences into the genome of Ff, we were able to convert single strand genome of Ff into double strand DNA structures. The presence of self complementary sequences in phage genome did not affect viral yields significantly, and the formation of double strands structures was successfully determined by a Mung Bean Nuclease resistance assay. Upon transfection into HEK293 cells, the double strand DNA structures showed to be readable by the transcriptional machinery of mammalian cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 8, August 2007, Pages 1081-1084
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 8, August 2007, Pages 1081-1084
نویسندگان
Y. Prieto, O. Sánchez,