کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1948648 | 1054705 | 2007 | 9 صفحه PDF | دانلود رایگان |

Activated mast cells play an important role in the development and maintenance of chronic inflammation by releasing proinflammatory cytokines such as Tumor necrosis factor alpha (TNFα). TNFα is a key mediator of immune and inflammatory responses as it controls the expression of inflammatory genes network and its overproduction contributes significantly to the pathological complications observed in many inflammatory diseases. We have previously shown that thymoquinone (TQ), which has broad anti-inflammatory activities, attenuates allergic inflammation in mice. In the present study, we investigated the effect of TQ on LPS-induced TNFα production in the rat basophil cell line, RBL-2H3. Stimulation of RBL-2H3 cells with LPS markedly increased TNFα production. TQ treatment significantly inhibited LPS-induced TNFα mRNA expression and protein production. To understand the mechanism by which TQ inhibited TNFα production, we examined its effects on activation of NF-κB transcription factor, which has been shown to be involved in regulating TNFα responses. LPS activated the NF-κB pathway, resulting in accumulation of NF-κB p65 and p50 subunits in the nucleus and activation of TNFα promoter. TQ administration to LPS-stimulated cells did not noticeably alter NF-κB cytosolic activation or nuclear expression as demonstrated by western blot analysis. Instead, TQ significantly increased the amount of the repressive NF-κB p50 homodimer, and simultaneously decreased the amount of transactivating NF-κB p65:p50 heterodimer, bound to the TNFα promoter as revealed by electrophoretic mobility shift and chromatin immunoprecipitation assays. Transient transfection of RBL-2H3 cells with TNFα promoter-driven luciferase gene constructs demonstrated that one of the three NF-κB binding sites in the TNFα promoter, the κB3 site, played a major role in the induction of TNFα promoter-driven luciferase gene expression by LPS, as well as in mediating the inhibitory effects of TQ on TNFα production, as TQ had minimal effect on the TNFα promoter-luciferase construct that lacks the κB3 site. Together, these results suggest that TQ attenuates the proinflammatory response in LPS-stimulated mast cells by modulating nuclear transactivation of NF-κB and TNFα production.
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 4, April 2007, Pages 556–564