The mammalian ABC transporter ABCA1 induces lipid-dependent drug sensitivity in yeast

► Successful expression and targeting in yeast of the murine ABCA1 transporter. ► Conservation of ABCA1 functional features across species (yeast to mouse). ► Definition of a novel ABCA1-specific compartment in yeast membrane. ► Induction of drug sensitivity by the expression of wild type ABCA1 in yeast. ► Interplay between ABCA1 function and membrane lipids in yeast and mammalian cells.