کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1949445 1537757 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dynamics of arachidonic acid mobilization by inflammatory cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Dynamics of arachidonic acid mobilization by inflammatory cells
چکیده انگلیسی

The development of mass spectrometry-based techniques is opening new insights into the understanding of arachidonic acid (AA) metabolism. AA incorporation, remodeling and release are collectively controlled by acyltransferases, phospholipases and transacylases that exquisitely regulate the distribution of AA between the different glycerophospholipid species and its mobilization during cellular stimulation. Traditionally, studies involving phospholipid AA metabolism were conducted by using radioactive precursors and scintillation counting from thin layer chromatography separations that provided only information about lipid classes. Today, the input of lipidomic approaches offers the possibility of characterizing and quantifying specific molecular species with great accuracy and within a biological context associated to protein and/or gene expression in a temporal frame. This review summarizes recent results applying mass spectrometry-based lipidomic approaches to the identification of AA-containing glycerophospholipids, phospholipid AA remodeling and synthesis of oxygenated metabolites.


► Cellular utilization of arachidonic acid.
► Methods and strategies for the analysis of arachidonic acid-containing lipids.
► Lipidomic studies of cellular arachidonate metabolism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1821, Issue 2, February 2012, Pages 249–256
نویسندگان
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