Apo J/clusterin expression and secretion: Evidence for 15-deoxy-Δ12,14-PGJ2-dependent mechanism

► Direct modulation of clusterin expression by Cox-2. ► Pre-secretory/secretory and pre-nuclear clusterin isoforms upregulated. ► Cox-2-dependent clusterin was normally processed and secreted. ► Cox-2 induced clusterin via activation of HSF1 and PPARγ. ► Cox-2/15d-PGJ2, but not clusterin, inhibited NF-κB signalling at the nuclear level.