کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1949500 1537760 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mass spectrometric analysis of neutral sphingolipids: Methods, applications, and limitations
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mass spectrometric analysis of neutral sphingolipids: Methods, applications, and limitations
چکیده انگلیسی

Sphingolipids represent an important class among lipids, especially when considering their vital roles in lipid metabolism. Thus, a variety of methods have been created to accomplish their analysis and the term “sphingolipidomics” has recently been coined to underline the motivation to enable a comprehensive analysis of all sphingolipid species including the acidic and the neutral ones. In this review, we summarize selected mainly biomedical based mass spectrometric approaches for the analysis of neutral sphingolipids regarding their advantages, applications and limitations. To underline some practical aspects of method development, we focus on a new method recently developed in our laboratory, which enables separation, detection, and mass spectrometric profiling of ceramide, hexosylceramide, lactosylceramide, globotriaosylceramide, globotetraosylceramide, sphingomyelin species, and cholesterol in one run. This method can be applied to investigate impairments of neutral sphingolipid metabolism in a variety of disorders such as sphingolipidoses and be employed to screen for sphingolipid profile changes as induced by knockout experiments or related studies. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrometry.


► Mass spectrometric methods for the analysis of neutral (glyco)sphingolipids.
► Focus is on ESI-MS-, APCI-MS-, and MALDI-MS- methods used in biomedical research.
► Shotgun methods in comparison to methods with pre-separations.
► Scope and limitations of glycosphingolipid and sphingolipid analysis by LC/APCI-MS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1811, Issue 11, November 2011, Pages 854–860
نویسندگان
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