کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1949501 1537760 2011 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-performance thin-layer chromatography/mass spectrometry for the analysis of neutral glycosphingolipids
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
High-performance thin-layer chromatography/mass spectrometry for the analysis of neutral glycosphingolipids
چکیده انگلیسی

This mini-review summarizes the protocol we have developed for the analysis of neutral glycosphingolipids (GSLs) by high-performance thin layer chromatography (HPTLC)–mass spectrometry (MS). We also present results obtained using this glycolipidomic approach to study neutral GSLs from mouse kidney, spleen, and small intestine. Finally, we discuss what is required for further development of this method, as well as what is expected for the future of glycolipid biology. This article is part of a Special Issue entitled Lipodomics and Imaging Mass Spectrometry.


► The protocol for the analysis of neutral glycosphingolipids (GSLs) by high-performance thin layer chromatography (HPTLC)-mass spectrometry (MS).
► Mass spectra of neutral GSLs prepared from mouse kidney, spleen, and small intestine.
► Coupling of the bird eye overview of GSLs by HPTLC with precise structural information by MS.
► Discussion on other MS methods for GSLs and further development of HPTLC-MS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1811, Issue 11, November 2011, Pages 861–874
نویسندگان
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