Lipid analysis and lipidomics by structurally selective ion mobility-mass spectrometry

Recent advances in mass spectrometry approaches to the analysis of lipids include the ability to incorporate both lipid class identification with lipid structural information for increased characterization capabilities. The detailed examination of lipids and their biosynthetic and biochemical pathways made possible by novel instrumental and bioinformatics approaches is advancing research in fundamental cellular and medical studies. Recently, high-throughput structural analysis has been demonstrated through the use of rapid gas-phase separation on the basis of the ion mobility (IM) analytical technique combined with mass spectrometry (IM-MS). While IM-MS has been extensively utilized in biochemical research for peptide, protein and small molecule analysis, the role of IM-MS in lipid research is still an active area of development. In this review of lipid-based IM-MS research, we begin with an overview of three contemporary IM techniques which show great promise in being applied towards the analysis of lipids. Fundamental concepts regarding the integration of IM-MS are reviewed with emphasis on the applications of IM-MS towards simplifying and enhancing complex biological sample analysis. Finally, several recent IM-MS lipid studies are highlighted and the future prospects of IM-MS for integrated omics studies and enhanced spatial profiling through imaging IM-MS are briefly described. This article is part of a Special Issue entitled Lipodomics and Imaging Mass Spectrometry.

► A review of ion mobility-mass spectrometry (IM-MS) for the study of lipids.
► IM-MS data dimensionality and reduces spectral complexity.
► IM-MS is used as an enhanced separation technique and as a structural analysis tool.
► Imaging IM-MS has potential high impact for analyzing complex biological samples.
► Contemporary examples of IM-MS lipid research are limited, but are rapidly expanding.