کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1950884 1055720 2011 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Somatostatin receptor-3 mediated intracellular signaling and apoptosis is regulated by its cytoplasmic terminal
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Somatostatin receptor-3 mediated intracellular signaling and apoptosis is regulated by its cytoplasmic terminal
چکیده انگلیسی

In the present study, we describe the role of cytoplasmic terminal (C-tail) domain in regulating coupling to adenylyl cyclase, signaling, and apoptosis in human embryonic kidney (HEK-293) cells transfected with wild type (wt)-hSSTR3 and C-tail deleted mutants. Cells transfected with wt-hSSTR3 and C-tail mutants show comparable membrane expression; however, display decreased expression in presence of agonist. wt-hSSTR3 exists as preformed homodimer at cell surface in basal conditions and decreases in response to agonist. Cells expressing C-tail mutants also show evidence of homodimerization with the same intensity as wt-hSSTR3. The agonist-dependent inhibition of cyclic adenosine monophosphate (cAMP) was lost in cells expressing C-tail mutants. Agonist treatment in cells expressing wt-hSSTR3 resulted in inhibition of cell proliferation, increased expression of PARP-1, and TUNEL positivity in proliferating cell nuclear antigen (PCNA)-positive cells. The agonist mediated increase in membrane expression of protein tyrosine phosphatase (PTP) seen with wt-hSSTR3 was diminished in C-tail mutants, which was accompanied with the loss of receptor's ability to induce apoptosis. Taken together, our data provide new insights into C-tail-dependent regulation of cell signaling and apoptosis by hSSTR3.

Research Highlights
► wt-hSSTR3 exists as homodimer at cell surface and decreases in response to agonist.
► cAMP inhibition by agonist was lost in C-tail mutants when compared to wt-hSSTR3.
► Cells expressing wt-hSSTR3 were positive to TUNEL and PCNA upon agonist treatment.
► Agonist-induced apoptosis was lost in cells expressing C-tail mutants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1813, Issue 3, March 2011, Pages 390–402
نویسندگان
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