Proteomic analysis for early neurodegenerative biomarker detection in an animal model

• Environmental etiology of neurodegeneration is associated with early exposure to xenobiotics.
• Long chain fatty acid transport protein resulted significantly decreased.
• Early deficit of plasma transthyretin and transferrin can be associated with the neurodegeneration.

The exposure to xenobiotics in the early stages of life represents the most important component in the etiology of many neurodegenerative disorders.Proteomic analysis of plasma and brain samples from early life treated animal model was performed in order to identify early biomarkers of neurodegeneration. Two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry identified four proteins in the plasma of adolescent rats that deviated from the control group. Low expression levels of transthyretin and plasma transferrin, and the absence of long-chain fatty acid transport 1 were measured. On the other hand, the same proteomic approach was done on striatum of an adult rat model of neurodegeneration. Mitochondrial aspartate aminotransferase and voltage-dependent anion channel were under expressed, while mitochondrial malate dehydrogenase, myelin basic protein and ubiquitin-60S ribosomal protein L40 were absent in striatum of animal model compared to control group. Data show that early biomarkers for the diagnosis of neurodegeneration can be obtained by proteomic analysis, starting from adolescent age and the results highlight the time frame for the onset of neurodegeneration due to early exposure to xenobiotics.

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